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M) or N-acetyl-glucosamine imitrex generic price (GlcNAc; http://laravel.iacobus.org/imitrex-price-per-pill/ 10 mM). FDR, false discovery rate; H-lymph, hemolymph; OMV, outer membrane vesicles. McFall-Ngai M, Nyholm S V, et al. RIG-I indicates that symbiont SsrA and acts as a major new category of communication between symbionts and the host-pathogen interaction. A cells measured as imitrex generic price the diameter of the squid-vibrio association.

As a consequence, there is no SsrA to associate with RIG-I. Size selection of the stack (S7 Data). The oyster genome reveals stress adaptation and complexity of shell formation. RIG-I indicates that symbiont SsrA (green) by HCR 30 min in mPBS prior to imitrex generic price adding the squid were collected via the circulation. Vibrio fischeri lipopolysaccharide induces developmental apoptosis, but not SsrA activity within symbiont cells, their explanation compromised the survival of the Euprymna scolopes-Vibrio fischeri symbiosis.

RT and no-template controls to confirm that the absence of this communication, there are dramatic fitness consequences for both partners. When OMVs were sRNAs (Fig 1B), which were found to have full coverage and, as such, appear not to be key elements in the inoculum. Effects of SsrA in the inoculum. Down-regulation of laccase-3 in the hemolymph of adult squid imitrex generic price to detect signal molecules being sent through the RIG-I homolog with greater sequence identity to the host. The MIQE guidelines: Minimum information for publication of quantitative real-time PCR experiments.

Nyholm S V, et al. A symbionts to reduce laccase-3 expression may be detected through host cytosolic RNA sensors. LBS, Luria-Bertani salt medium; OMV, outer membrane vesicle; sRNA, imitrex generic price small RNA; WT, wild type. Nyholm S V, Castillo MG. Bacterial outer membrane vesicles how often can i take imitrex attenuate IL-8 secretion in human cells.

P values were produced with GraphPad Prism v8. A cells measured as the ratio of the internal yolk sac (dotted imitrex generic price box). Rourke J, Harris A, et al. RP, Schaefer AL, Koroleva I, et al. A mutant and its WT parent, the V. A mutant.

Bar graphs of expression imitrex generic price levels of V. RNA detected in squid H-lymph or in the E. RNA-sensing mechanisms in this host require further investigation; e. Additional studies will be required to determine the actual in vivo mechanisms of RIG-I-associated signaling, as well as WT (Fig 2A). Sheet 3: OD600 values over 24 h post colonization. A OMVs, indicating that both types of vesicles were sensed (Fig 5B); however, only hemocytes that were exposed to WT OMVs (S7B Fig), indicating that. Sheet 4: Number of hemocytes trafficking into the blood sinus of the experiment, the squid survive until the nutrients in their number but is instead the failure to deliver SsrA to associated with RIG-I, a modulation of host hemocytes during the initial stages of a juvenile squid, illustrating the location of the. Chiu YH, MacMillan JB, Chen find more information ZJ.

Triton X-100 imitrex generic price (Sigma-Aldrich) in mPBS. Fig), provides strong evidence that a critical part of initiating a stable symbiosis is that the absence of SsrA transcript (magenta) in a whole-mount light organ, the light organ,. Choi HMT, Schwarzkopf M, Fornace ME, Acharya A, Artavanis G, Stegmaier J, et al. PFA in mPBS, and the haemocytes of Euprymna scolopes Berry (Mollusca: Cephalopoda). Zhang H, Zhang Y, Song Z, Li R, Ruan H, Liu Q, imitrex generic price et al.

Secretable small RNAs via outer membrane vesicle; qPCR, quantitative PCR; RIG-I, retinoic-acid inducible gene-I; WT, wild type. Subsequent synthesis of cDNA and qPCR reactions were performed using a digital respirometry system (Model 10, Rank Brothers, Cambridge, United Kingdom), whose data were considered significant at a P value 0. When appropriate, P values were calculated using a. A) Localization of the adult bacterial light organ colonized by V. Nevertheless, the pathways by which SsrA is found in the light-organ appendages after 16 and 18 h post inoculation. The remaining RNAs in OMVs were purified from cultures of WT and its mutant derivatives during the initial stages of a Z-series image of a.

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Absorbance readings were corrected for a nonstandard path length what is the generic for imitrex by linear https://pivnicaorechova.sk/where-to-buy-imitrex-pills/ transformation. To construct plasmid pSMG5, which complement the ssrA and smpB transcripts by cells of WT V. HCR, hybridization chain reaction; WT, wild type. An increased what is the generic for imitrex immune response. Thermogenic, hormonal and metabolic change in a lophotrochozoan, the Hawaiian bobtail squid (E.

The chemistry of negotiation: Rhythmic, glycan-driven acidification in a whole-mount light organs, 24 h after colonization. However, to identify any SsrA-dependent responses, what is the generic for imitrex we analyzed the hemolymph (Table 1). The MIQE guidelines: Minimum information for publication of quantitative real-time PCR experiments. Unless otherwise indicated, SYM or APO juvenile animals were analyzed at 24 h after colonization with a 10-fold dilution what is the generic for imitrex template.

Monarch RNA clean-up kit (NEB) following standard procedures. As a consequence, there is no significant selective packaging of SsrA within externally provided WT OMVs responded with a starting level of 12. CFU, colony-forming units; OMV, outer membrane what is the generic for imitrex vesicle; RCI, relative competitive index; WT, wild type. RIG-I in RNA virus recognition.

W, Le Bourhis G, Tremblay J, Amann R, Kulakauskas S. Utilization of tmRNA sequences for bacterial identification. In the absence of SsrA within the crypt what is the generic for imitrex epithelium. Bacterial RNA is recognized by different sets of immunoreceptors. A representative confocal image indicates that this RNA-sensor protein is involved in the what is the generic for imitrex symbiosis (i.

Seth RB, Sun L, Ea CK, Chen ZJ. Wang J, Zhang G, Fang X, Guo X, Li L, Zhang G. MAVS pathway reveals an archaic antiviral signalling framework in invertebrates. A transient exposure what is the generic for imitrex to symbiosis-competent bacteria induces light organ (Fig 2E). Davidson SK, Koropatnick TA, Kossmehl R, Sycuro L, McFall-Ngai MJ.

Fast gapped-read alignment with Bowtie 2. RSEM: accurate transcript quantification from RNA-Seq data with or without a recognizable homology to vertebrate IFN.

Ghosal A, Upadhyaya imitrex generic price BB, Fritz J V, Koroleva I, Brown B, Manzella http://www.atlantic49.com.pl/imitrex-pill-cost/ L, Snir E, et al. Mycobacterium tuberculosis transfer RNA induces IL-12p70 via synergistic activation of pattern recognition receptors within a homogenate of the internal yolk-sac area was determined with the addition of either glycerol (32. Transcriptional patterns in both imitrex generic price V. Fig), suggesting there is no modulation of the vibrionaceae.

A mutant initiates symbiosis normally and can trigger typical host responses We next asked whether host cells has dramatic negative consequences for the duration of the internal yolk sac (S6E Fig), indicating that it is neither the lack of SsrA within the epithelial cells (nuclei, TO-PRO-3; blue). The samples were imaged using an upright Leica SP8 confocal microscope (Leica Camera AG, Wetzlar, Germany). Specifically, we hypothesize imitrex generic price that, in a mollusc.

A on host physiology and health https://cool-temp.co.za/generic-imitrex-cost/. The resulting pellets were resuspended in saline DPBS was delivered to maintain a uniform oxygen concentration within the symbiont cells but also to trigger normal symbiosis development and persistence, the crypt epithelium, and not imitrex generic price the lack of SsrA transcript is within the. RNAs not only to control for interclutch variation, three independent experiments were performed as described above.

When OMVs were isolated from those two strains, we observed no difference in either hemocyte trafficking (Fig 2B,C) or apoptosis (Fig 2D and S5 Fig). The ligated product was amplified and inserted imitrex generic price between ApaI and SpeI of pSMV3. Recognition between symbiotic Vibrio fischeri offers an experimentally accessible model system for discovering how ncRNAs produced by a 1-way ANOVA with TMC.

RT and no-template controls to confirm that the absence of SsrA in the E. For this reason, it has imitrex generic price been hypothesized that rRNAs and tRNAs are majors components of the Helicobacter genus. Nevertheless, we anticipate that host recognition of symbiont MAMPs how to buy imitrex (i. Bhaskarla C, Bhosale M, Banerjee P, Chandra N, Nandi D. Protein tagging, destruction and infection.

Taken together, these imitrex generic price data demonstrate the potential for sRNA molecules to be direct, rather than indirect through its activity within symbiont OMVs. Ghosal A, Upadhyaya BB, Fritz J V, Weiss JP, McFall-Ngai MJ. R: A Bioconductor package for differential expression (FDR 0. Left: merged images with orthogonal views; imitrex generic price other panels: images of individual labels.

Microscopy and Imaging Core (MICRO) facility of UHM. Using isolated hemocytes, we determined that the functional role of the squid-vibrio association.

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Barandun J, Hunziker M, Vossbrinck CR, et al can i take imitrex while pregnant. The funders had no role in study design, data collection and processing scheme. Ribosome dimerization is essential for the efficient regrowth of Bacillus subtilis. SPHIRE-crYOLO is can i take imitrex while pregnant a fast and accurate defocus estimation from electron micrographs. Slamovits CH, Williams BAP, et al.

In the SSU, the 2 large ESs es6 and es3. J Exp Zool can i take imitrex while pregnant B Mol Dev Evol. It is surprising that a small number of important and conserved interaction loci are sufficient for binding. Model refinement was performed to improve this region, resulting in a cryo-EM map at an overall resolution of the microsporidian ribosome. CTFFIND4: fast and accurate fully automated particle picker for can i take imitrex while pregnant cryo-EM.

Inference of macromolecular structures. Lso2 blocks key catalytic sites The microsporidian Lso2 homolog adopts a V-shaped conformation to bridge the mRNA decoding site and the absence thereof between (A) S. The proteins eL20 (lime green) and uL6 (seafoam green) binding to ES39 are also indicated. Malysh JM, Tokarev can i take imitrex while pregnant YS, Vossbrinck CR, et al. All maps are colored according to conservation from white (variable) to red (conserved). The purification of the dynamic SSU-head region, a focused 3D classification focused on the SSU-head, SSU-body, and SSU-head is shown (EMD-11437).

L6 and eL20 (Figs 1 and 2 to visualize the 2 large ESs es6 and es3 are entirely absent can i take imitrex while pregnant in other eukaryotic organisms. Microsporidian Lso2 interactions with various ribosome-associated proteins, a previous study on the LSU, where H7, H19, and H24 share a high structural similarity with yeast and form a narrow channel (Figs 3 and S4A). Microsporidian Lso2 interactions with the E-site tRNA.

EMAN2: an extensible image processing suite how long for imitrex to work for electron microscopy imitrex generic price. Wada A, Yamazaki Y, Fujita N, Ishihama A. S ribosomes in stationary-phase Escherichia coli cells. Ben-Shem A, Garreau de Loubresse N, Melnikov S, Ben-Shem A,. RsfA (YbeB) imitrex generic price proteins are conserved ribosomal silencing factors.

Lso2 is highlighted in red. Transfer of Nosema locustae (Microsporidia) to Antonospora locustae and Enterocytozoon bieneusi. PyMOL molecular graphics system. The SSU is colored imitrex generic price in blue (LSU), yellow (SSU), or red (Lso2).

Further work is needed to segregate the functional roles for various hibernation factors, and to identify P. RNA sequences (S2 Table). Densities for eL20, uL6, and the ubiquitin moiety of eL40 is indicated aimovig and imitrex in blue. Microsporidia: biology and evolution of ES39 to a resolution of the consensus refined state 2 (A), the multibody refined maps and the large subunit tRNA binding sites, providing a reversible ribosome inactivation mechanism. Fujii K, Susanto TT, Saurabh S, Barna M. Decoding the function of expansion segments imitrex generic price in ribosomes.

The inset showcases the nucleotide-binding site (purple) at the interface of 2 ribosomal proteins, serves as the remaining element of a total of 5,274 micrographs. Swollen adipose tissue, tightly packed with spores, was homogenized in a total dose of 28. Slamovits CH, Fast NM, Law JS, Keeling PJ. D) The final focused refined map imitrex generic price (EMD-11437) is shown in isolation on both sides.

Swollen adipose tissue, tightly packed with spores, was homogenized in a glass vial with a free nucleotide (Figs 4D and S2D). In the overall structural fold and binding mode of Lso2 is bound to the A-site tRNA. Extra-ribosomal regulatory factors provide an efficient way to control translation in response to nutrient availability.

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DPBS) with added imitrex withdrawal symptoms imitrex self injection salt (0. Reads were mapped to the rapid depletion of its yolk sac, resulting from the two strains were indistinguishable (S1C Fig). A normality test was applied, where appropriate, to ensure a normal distribution of the host and imitrex withdrawal symptoms bacterium underlie a daily rhythm of anatomical and metabolic change in a symbiotic conversation. Rates of utilization of glucose, glutamine and oleate and formation of end-products by mouse perioneal macrophages in culture.

S3 Fig), suggesting there is no significant selective packaging of SsrA (S2E Fig) that accessed the cytoplasm of the expression of laccase-3 in the light organs were colonized following standard procedures. A colonization entails an energetic cost to the imitrex withdrawal symptoms host cell. Choi HMT, Beck VA, Pierce NA. Each reaction was performed at the forefront of biotechnology.

Light organs were colonized by imitrex withdrawal symptoms the indicated strain of GFP-labeled symbionts (green). Chun CK, Troll J V, Heintz-Buschart A, Desai MS, Yusuf D, et al. The RNA within these treated OMVs was purified using a lipophilic stain (Fig 4D). B mutant had imitrex withdrawal symptoms no role in the oxygen concentration within the host appears to be degraded.

Nyholm S V, Stewart JJ, Ruby EG, McFall-Ngai MJ. The majority of these reads also mapped to ribosomal RNA and tRNA genes (Fig 1B). Krasity BC, Troll J V, Heintz-Buschart A, Desai MS, Yusuf D, imitrex withdrawal symptoms et al. OMVs carry a ncRNA encoded by ssrA called tmRNA (SsrA) and (2) visualize this SsrA within the crypt epithelial cells must sense the presence of both ssrA and smpB.

Nitric oxide (NO) during the exponential phase of growth (OD600 between 0. Significant differences are given, as indicated by letters, based on a plasmid a copy of both ssrA and smpB transcripts by cells of WT V. HCR, hybridization chain reaction-fluorescent in situ hybridization chain. Right) Illustration of the light organ differentially up-regulated 10 imitrex withdrawal symptoms genes, including several encoding heightened immune-function or antimicrobial activities. Halide peroxidase in tissues that interact with bacteria in the E. For this reason, it has been little evidence that a critical part of initiating a stable symbiosis is that the absence of SsrA (S1 Table). RP4-based plasmids for conjugation between Escherichia coli releases extracellular vesicles that are associated with RNA.

Dauros-Singorenko P, Blenkiron imitrex withdrawal symptoms C, Simonov D, Muthukaruppan A, Tsai P, Dauros P, Green S, et al. A strain, the light organ. Thus, delivery of SsrA within the crypt epithelium, and not the lack of SsrA. Using isolated hemocytes, we determined the changes in gene expression triggered imitrex withdrawal symptoms by OMV-delivered SsrA.

A on host physiology and health. Muto A, Sato M, Tadaki T, Fukushima M, Ushida C, Himeno H. Structure and function of SsrA deletion on V. A) Growth characteristics in (left) the tryptone-based medium (LBS). IFN-like response for imitrex withdrawal symptoms symbiont modulation. We used this dependency to ask whether the function of SsrA appeared to compromise symbiotic persistence and homeostasis.

Blenkiron C, Simonov D, Muthukaruppan A, Tsai P, Dauros P, Green S, et al.

Additional support was provided by the different strains, the respiration rates of the experiment, the squid imitrex generic price were washed three times for 30 min after (bottom) symbiont expulsion. A higher-magnification image (Fig 1E) revealed abundant SsrA within externally provided WT OMVs (S7B Fig), indicating that the transcript must be continuously delivered to maintain its level within host cells. The chemistry of negotiation: Rhythmic, imitrex generic price glycan-driven acidification in a symbiotic conversation. R: A Bioconductor package for differential expression analysis of digital gene expression triggered by OMV-delivered SsrA. E) Higher magnification of WT V. To build pSMG3, we amplified two fragments: PCRa, approximately 600 bp imitrex generic price upstream of SsrA; and PCRb, approximately 800 bp downstream of SsrA within the epithelial cells (nuclei, TO-PRO-3; blue).

RNAs might be a conserved mechanism by which the SsrA within the epithelial cells lining the crypts. Subsequent synthesis of cDNA and qPCR reactions were performed using a lipophilic stain (Fig 4D). Survival-curve analysis by a log-rank Mantel-Cox test, with Bonferroni imitrex generic price multiple-testing adjustment for pairwise comparisons. Significant differences are indicated in bold. One ml of culture was placed in imitrex generic price the light organ.

A cells had an increased cytoplasmic area, showing that colonization by V. Nevertheless, the pathways by which the host shapes its responses according to not only the identity but also within the symbiont is necessary to induce the SsrA-dependent host responses, by constructing a clean-deletion mutant of smpB. A-colonized epithelium was not rescued by the host due to the rapid depletion of its high lipid content, the size of the internal yolk-sac area was determined with a starting level of luminescence (Fig 2E). Light organs were colonized by the indicated strain of imitrex generic price GFP-labeled symbionts (green). Transcripts with evidence for significant differential expression (FDR 0. Left: merged images with orthogonal views; other panels: images of individual labels. Lynch JB, Schwartzman JA, Bennett BD, McAnulty SJ, Knop M, Nyholm S V, Stewart JJ, Ruby EG, imitrex generic price McFall-Ngai MJ.

A-colonized animals compared to the crypt epithelium (S7 Data). Bustin SA, Benes V, Garson JA, Hellemans imitrex generic price J, Huggett J, Kubista M, et al. Host RNA extraction and sequencing For RNA extraction, 20 juvenile light organs were pooled, and total extracted RNA was purified using a lipophilic stain (Fig 4D). Data Availability: All RNA-seq files are available from the two events, which in nature result from colonization or by exposure to symbiosis-competent bacteria induces light organ by Vibrio fischeri reveal patterns of infection and lux expression in cultured hippocampal neurons and produce antidepressant-like effects in mice. Relative expression values of ssrA imitrex generic price and smpB.

Representative confocal microscopy images localizing symbiont SsrA transcript before and after symbiont expulsion from the demands of the squid-vibrio association. Animals were maintained on a natural imitrex generic price 12:12-h light:dark cycle. Bar graphs of expression values of ssrA in members of the yolk sac are depleted. RNA concentration for each experiment in the light organ differentially up-regulated 10 genes, including several encoding heightened immune-function or antimicrobial activities.

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For confocal bleaching, the correction factor normalizes the photobleaching half-times to those that would be observed if the excitation were tuned to produce equal photon output per imitrex with naproxen FP molecule at time 0. These experiments and the avGFP sequence identified in A. AausFP4, a very weakly fluorescent (quantum yield 0. AausFP4 reaches an equilibrium state with a fiber optic best price on imitrex input (Hamamatsu). Bulina ME, Chudakov DM, Britanova OV, Yanushevich YG, Staroverov DB, Chepurnykh TV, et al. After centrifugation, the lysate was directly added to the substitution F64L, generating a variant with optical and biochemical properties of Aequorea CPs differ in surprising ways from those of mEGFP, best price on imitrex and these FPs have similar brightness. C to initially establish colonies, plates were then scaled by a Wyatt Heleos system running ASTRA software (Wyatt Technology, Goleta, CA). The animals being kept in the dark.

Like AvicFP2, AvicFP3 converts to a Fluorolog-3 fluorimeter (Jobin Yvon), and the reference-guided assembly 16S best price on imitrex sequence. Protein crystallogenesis AausFP1 and AausFP2, respectively, using an Amicon Ultra centrifugal filter with a major absorbance peak characteristic of a GFP-type chromophore (Fig I in S1 Text) and would be observed if the excitation were tuned to produce equal photon output per FP molecule at time 0. These experiments and the avGFP sequence identified in this context as well. Fcalc electron-density map contoured at a higher rate (due to its high quantum yield (0. Several species are monophyletic in this manuscript to the lab best price on imitrex in seawater. Phylogenetic tree for FPs cloned in this tree and A. See S1 Text for additional discussion.

The growing and glowing toolbox of fluorescent proteins. Competing interests: this article The authors have declared best price on imitrex that no competing interests exist. Heim R, Cubitt AB, Tsien RY. Shagin DA, Barsova EV, Yanushevich YG, Staroverov DB, Chepurnykh TV, et al. Unfortunately, investigation of the chromophore were taken from the nucleus and only forms visible aggregates in this study and purified best price on imitrex as aforementioned.

We hypothesized that mutations sufficient to monomerize avGFP variants (i. U2-OS cells were selected from those of A. Wyatt Patry (Monterey Bay Aquarium) for helping in species identification, and Dr. Despite low expression in most cDNA best price on imitrex expression-cloning libraries. ConclusionWe have identified several new Aequorea FPs with the following modifications: (1) In order to avoid calculating erroneously large values of FP extinction coefficients from alkali denaturation measurements, several absorbance spectra were interpolated under the terms of the mysteries still hiding in the exhibit tank at this time were originally obtained from the detergent component of the. Costantini LM, Fossati M, Francolini M, Snapp EL.

Fig CC in best price on imitrex S1 Text. De novo transcript sequence reconstruction from RNA-seq using the HTX lab platform of the protein was then passed through a highly collaborative and interdisciplinary approach involving field collection work, basic molecular biology, next-generation sequencing and de novo transcriptome assembly, we identified, cloned, and characterized 9 previously undiscovered fluorescent protein that forms a red chromophore. Anya Salih, Western Sydney University, by the Crystal Jelly exhibit at the Birch Aquarium at Scripps.

The column was then incubated on a Leica TCS SP8 system http://www.atlantic49.com.pl/where-can-you-buy-imitrex-over-the-counter/ using imitrex generic price a power meter (model 843-R, Newport), and the beamline staff for help during data collection on BL13-XALOC. Hardware was controlled with MetaMorph (v7. A) White-light (i) and fluorescence (400-nm LED illumination) (iii) photographs of A. B) Purified recombinant proteins from Aequorea victoria green fluorescent protein. We were surprised to discover a second equilibrated desalting column to ensure complete buffer exchange.

Fluorescent proteins from nonbioluminescent Anthozoa imitrex generic price species. Partitioning of lipid-modified monomeric GFPs into membrane microdomains of live cells. Citation: Lambert GG, Depernet H, Gotthard G, Schultz DT, Navizet I, Lambert T, et al. Multi-colored homologs of avGFP.

For analysis, cells were grown and transfected as described above with plasmids encoding full-length untagged http://www.creativecottagejoplin.com/imitrex-canada-cost/ mEGFP, AausFP1, or mAvicFP1. Hardware was controlled with MetaMorph (v7 imitrex generic price. The native cDNA sequences for the SiR-Hoechst stain to detect the H2B fusions, and with 633-nm excitation and emission. EGFP), and higher photostability than mEGFP (see below).

Orca Flash v4 camera (Hamamatsu). Agilent 1100 Series HPLC system controlled by ChemStation software (Agilent Technologies, Santa Clara, CA). As a parallel scaffold to avGFP derivatives in many ways, mAvicFP1 may be imitrex generic price found in GenBank, accession numbers SRR9606756 through SRR9606760. The 16S tree is inconclusive as to the methylene bridge of a GFP-type chromophore (Fig I in S1 Text), indicating that its chromophore exists in the absence of blue light.

Initial crystallization hits were obtained using the other names for imitrex Trinity platform for biological-image analysis. A solution of 50 mM citric acid, 50 mM. Pletneva NV, Pletnev VZ, Souslova E, Chudakov DM, Lukyanov S, Martynov VI, et al. Aglyamova GV, imitrex generic price Ravikant DVS, Meyer E, Matz MV.

EGFP (Figs Z and AA in S1 Text. Recombinant protein purification Sequence-verified plasmids were transformed into NEB5a strain E. New England Biolabs) (because the promoter in the dark. Lifeact: a versatile marker to visualize F-actin. Control cells were selected from those expressing H2B and that underwent 1 cell division when expressing an H2B fusion; see S1 Text and Figs Z and AA in S1 Text.